PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Zika Virus RNA Replication and Persistence in Brain and Placental Tissue.

Abstract Zika virus is causally linked with congenital microcephaly and may be associated with pregnancy loss. However, the mechanisms of Zika virus intrauterine transmission and replication and its tropism and persistence in tissues are poorly understood. We tested tissues from 52 case-patients: 8 infants with microcephaly who died and 44 women suspected of being infected with Zika virus during pregnancy. By reverse transcription PCR, tissues from 32 (62%) case-patients (brains from 8 infants with microcephaly and placental/fetal tissues from 24 women) were positive for Zika virus. In situ hybridization localized replicative Zika virus RNA in brains of 7 infants and in placentas of 9 women who had pregnancy losses during the first or second trimester. These findings demonstrate that Zika virus replicates and persists in fetal brains and placentas, providing direct evidence of its association with microcephaly. Tissue-based reverse transcription PCR extends the time frame of Zika virus detection in congenital and pregnancy-associated infections.
PMID
Related Publications

Projecting Month of Birth for At-Risk Infants after Zika Virus Disease Outbreaks.

Notes from the Field: Evidence of Zika Virus Infection in Brain and Placental Tissues from Two Congenitally Infected Newborns and Two Fetal Losses--Brazil, 2015.

Increase in Reported Prevalence of Microcephaly in Infants Born to Women Living in Areas with Confirmed Zika Virus Transmission During the First Trimester of Pregnancy - Brazil, 2015.

Birth Defects Among Fetuses and Infants of US Women With Evidence of Possible Zika Virus Infection During Pregnancy.

Pathology of congenital Zika syndrome in Brazil: a case series.

Authors

Mayor MeshTerms
Keywords

RT-PCR

Zika virus

brain

formalin-fixed

in-situ hybridization

paraffin-embedded tissues

placenta

replication

vector-borne infections

viruses

Journal Title emerging infectious diseases
Publication Year Start




PMID- 27959260
OWN - NLM
STAT- Publisher
DA  - 20161213
LR  - 20161214
IS  - 1080-6059 (Electronic)
IS  - 1080-6040 (Linking)
VI  - 23
IP  - 3
DP  - 2017 Mar 15
TI  - Zika Virus RNA Replication and Persistence in Brain and Placental Tissue.
LID - 10.3201/eid2303.161499 [doi]
AB  - Zika virus is causally linked with congenital microcephaly and may be associated 
      with pregnancy loss. However, the mechanisms of Zika virus intrauterine
      transmission and replication and its tropism and persistence in tissues are
      poorly understood. We tested tissues from 52 case-patients: 8 infants with
      microcephaly who died and 44 women suspected of being infected with Zika virus
      during pregnancy. By reverse transcription PCR, tissues from 32 (62%)
      case-patients (brains from 8 infants with microcephaly and placental/fetal
      tissues from 24 women) were positive for Zika virus. In situ hybridization
      localized replicative Zika virus RNA in brains of 7 infants and in placentas of 9
      women who had pregnancy losses during the first or second trimester. These
      findings demonstrate that Zika virus replicates and persists in fetal brains and 
      placentas, providing direct evidence of its association with microcephaly.
      Tissue-based reverse transcription PCR extends the time frame of Zika virus
      detection in congenital and pregnancy-associated infections.
FAU - Bhatnagar, Julu
AU  - Bhatnagar J
FAU - Rabeneck, Demi B
AU  - Rabeneck DB
FAU - Martines, Roosecelis B
AU  - Martines RB
FAU - Reagan-Steiner, Sarah
AU  - Reagan-Steiner S
FAU - Ermias, Yokabed
AU  - Ermias Y
FAU - Estetter, Lindsey B C
AU  - Estetter LB
FAU - Suzuki, Tadaki
AU  - Suzuki T
FAU - Ritter, Jana
AU  - Ritter J
FAU - Keating, M Kelly
AU  - Keating MK
FAU - Hale, Gillian
AU  - Hale G
FAU - Gary, Joy
AU  - Gary J
FAU - Muehlenbachs, Atis
AU  - Muehlenbachs A
FAU - Lambert, Amy
AU  - Lambert A
FAU - Lanciotti, Robert
AU  - Lanciotti R
FAU - Oduyebo, Titilope
AU  - Oduyebo T
FAU - Meaney-Delman, Dana
AU  - Meaney-Delman D
FAU - Bolanos, Fernando
AU  - Bolanos F
FAU - Saad, Edgar Alberto Parra
AU  - Saad EA
FAU - Shieh, Wun-Ju
AU  - Shieh WJ
FAU - Zaki, Sherif R
AU  - Zaki SR
LA  - eng
PT  - Journal Article
DEP - 20170315
PL  - United States
TA  - Emerg Infect Dis
JT  - Emerging infectious diseases
JID - 9508155
OTO - NOTNLM
OT  - RT-PCR
OT  - Zika virus
OT  - brain
OT  - formalin-fixed
OT  - in-situ hybridization
OT  - paraffin-embedded tissues
OT  - placenta
OT  - replication
OT  - vector-borne infections
OT  - viruses
EDAT- 2016/12/14 06:00
MHDA- 2016/12/14 06:00
CRDT- 2016/12/14 06:00
AID - 10.3201/eid2303.161499 [doi]
PST - aheadofprint
SO  - Emerg Infect Dis. 2017 Mar 15;23(3). doi: 10.3201/eid2303.161499.

<?xml version="1.0" encoding="UTF-8"?>
<b:Sources SelectedStyle="" xmlns:b="http://schemas.openxmlformats.org/officeDocument/2006/bibliography"  xmlns="http://schemas.openxmlformats.org/officeDocument/2006/bibliography" >
</b:Sources>