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Post-injury stress signals alter epigenetic profile of cytosine methylation in the proviral genome of endogenous retroviruses.

Abstract The majority of epigenetic methylation events at cytosine residues of the genome are reported to occur in transposable elements, as a result, it contributes to genome stability by repressing their transposition activity. Our recent studies demonstrated that the expression of certain murine endogenous retroviruses (MuERVs), a family of retrotransposons, is modulated in the liver after burn injury and sepsis. In this study, we investigated whether burn-elicited stress signals alter epigenetic methylation profile of cytosine residues of the MuERV proviral genome. Female C57BL/6J mice were subjected to ~18% total body surface area burn. The genomic DNAs from the livers, which were collected at 3 and 24 h after burn, were treated with bisulfite to convert unmethylated cytosines (C) to thymines (T). From four experimental groups (no burn-3h, burn-3h, no burn-24h, and burn-24h), 91, 98, 94, and 86 unique U3 sequences (from sense or antisense strand) were cloned, respectively and a total of 16 different U3 sizes were identified among them. The survey of C to T conversions in these U3 sequences revealed that the epigenetic profiles of cytosine methylation are differentially affected (increase or decrease in demethylated cytosine residues) by stress signals from burn and/or anesthesia-resuscitation in a position of cytosine residue and/or size of U3 sequence-specific manner. In addition, the methylation characteristics of the majority of cytosine residues of the different U3 sequences within each size group were conserved. The findings from this study suggest that burn-elicited stress signals contribute to a transient or permanent alteration in cytosine methylation characteristics of certain MuERV loci in the genome, potentially modulating transcription activity of their own as well as neighboring genes.
PMID
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Authors

Mayor MeshTerms

Epigenesis, Genetic

Keywords
Journal Title experimental and molecular pathology
Publication Year Start
%A Chiu, Sophia; Hsu, Karen; Greenhalgh, David G.; Cho, Kiho
%T Post-injury stress signals alter epigenetic profile of cytosine methylation in the proviral genome of endogenous retroviruses.
%J Experimental and molecular pathology, vol. 89, no. 3, pp. 291-300
%D 12/2010
%V 89
%N 3
%M eng
%B The majority of epigenetic methylation events at cytosine residues of the genome are reported to occur in transposable elements, as a result, it contributes to genome stability by repressing their transposition activity. Our recent studies demonstrated that the expression of certain murine endogenous retroviruses (MuERVs), a family of retrotransposons, is modulated in the liver after burn injury and sepsis. In this study, we investigated whether burn-elicited stress signals alter epigenetic methylation profile of cytosine residues of the MuERV proviral genome. Female C57BL/6J mice were subjected to ~18% total body surface area burn. The genomic DNAs from the livers, which were collected at 3 and 24 h after burn, were treated with bisulfite to convert unmethylated cytosines (C) to thymines (T). From four experimental groups (no burn-3h, burn-3h, no burn-24h, and burn-24h), 91, 98, 94, and 86 unique U3 sequences (from sense or antisense strand) were cloned, respectively and a total of 16 different U3 sizes were identified among them. The survey of C to T conversions in these U3 sequences revealed that the epigenetic profiles of cytosine methylation are differentially affected (increase or decrease in demethylated cytosine residues) by stress signals from burn and/or anesthesia-resuscitation in a position of cytosine residue and/or size of U3 sequence-specific manner. In addition, the methylation characteristics of the majority of cytosine residues of the different U3 sequences within each size group were conserved. The findings from this study suggest that burn-elicited stress signals contribute to a transient or permanent alteration in cytosine methylation characteristics of certain MuERV loci in the genome, potentially modulating transcription activity of their own as well as neighboring genes.
%K Animals, Base Sequence, Burns, Cytosine, DNA Methylation, Endogenous Retroviruses, Epigenesis, Genetic, Female, Liver, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Polymerase Chain Reaction, Proviruses, Signal Transduction, Stress, Physiological
%P 291
%L 300
%Y 10.1016/j.yexmp.2010.06.012
%W PHY
%G AUTHOR
%R 2010.......89..291C

@Article{Chiu2010,
author="Chiu, Sophia
and Hsu, Karen
and Greenhalgh, David G.
and Cho, Kiho",
title="Post-injury stress signals alter epigenetic profile of cytosine methylation in the proviral genome of endogenous retroviruses.",
journal="Experimental and molecular pathology",
year="2010",
month="Dec",
day="06",
volume="89",
number="3",
pages="291--300",
keywords="Animals",
keywords="Base Sequence",
keywords="Burns",
keywords="Cytosine",
keywords="DNA Methylation",
keywords="Endogenous Retroviruses",
keywords="Epigenesis, Genetic",
keywords="Female",
keywords="Liver",
keywords="Mice",
keywords="Mice, Inbred C57BL",
keywords="Molecular Sequence Data",
keywords="Polymerase Chain Reaction",
keywords="Proviruses",
keywords="Signal Transduction",
keywords="Stress, Physiological",
abstract="The majority of epigenetic methylation events at cytosine residues of the genome are reported to occur in transposable elements, as a result, it contributes to genome stability by repressing their transposition activity. Our recent studies demonstrated that the expression of certain murine endogenous retroviruses (MuERVs), a family of retrotransposons, is modulated in the liver after burn injury and sepsis. In this study, we investigated whether burn-elicited stress signals alter epigenetic methylation profile of cytosine residues of the MuERV proviral genome. Female C57BL/6J mice were subjected to {\textasciitilde}18\% total body surface area burn. The genomic DNAs from the livers, which were collected at 3 and 24 h after burn, were treated with bisulfite to convert unmethylated cytosines (C) to thymines (T). From four experimental groups (no burn-3h, burn-3h, no burn-24h, and burn-24h), 91, 98, 94, and 86 unique U3 sequences (from sense or antisense strand) were cloned, respectively and a total of 16 different U3 sizes were identified among them. The survey of C to T conversions in these U3 sequences revealed that the epigenetic profiles of cytosine methylation are differentially affected (increase or decrease in demethylated cytosine residues) by stress signals from burn and/or anesthesia-resuscitation in a position of cytosine residue and/or size of U3 sequence-specific manner. In addition, the methylation characteristics of the majority of cytosine residues of the different U3 sequences within each size group were conserved. The findings from this study suggest that burn-elicited stress signals contribute to a transient or permanent alteration in cytosine methylation characteristics of certain MuERV loci in the genome, potentially modulating transcription activity of their own as well as neighboring genes.",
issn="1096-0945",
doi="10.1016/j.yexmp.2010.06.012",
url="http://www.ncbi.nlm.nih.gov/pubmed/20609362",
language="eng"
}

%0 Journal Article
%T Post-injury stress signals alter epigenetic profile of cytosine methylation in the proviral genome of endogenous retroviruses.
%A Chiu, Sophia
%A Hsu, Karen
%A Greenhalgh, David G.
%A Cho, Kiho
%J Experimental and molecular pathology
%D 2010
%8 Dec 06
%V 89
%N 3
%@ 1096-0945
%G eng
%F Chiu2010
%X The majority of epigenetic methylation events at cytosine residues of the genome are reported to occur in transposable elements, as a result, it contributes to genome stability by repressing their transposition activity. Our recent studies demonstrated that the expression of certain murine endogenous retroviruses (MuERVs), a family of retrotransposons, is modulated in the liver after burn injury and sepsis. In this study, we investigated whether burn-elicited stress signals alter epigenetic methylation profile of cytosine residues of the MuERV proviral genome. Female C57BL/6J mice were subjected to ~18% total body surface area burn. The genomic DNAs from the livers, which were collected at 3 and 24 h after burn, were treated with bisulfite to convert unmethylated cytosines (C) to thymines (T). From four experimental groups (no burn-3h, burn-3h, no burn-24h, and burn-24h), 91, 98, 94, and 86 unique U3 sequences (from sense or antisense strand) were cloned, respectively and a total of 16 different U3 sizes were identified among them. The survey of C to T conversions in these U3 sequences revealed that the epigenetic profiles of cytosine methylation are differentially affected (increase or decrease in demethylated cytosine residues) by stress signals from burn and/or anesthesia-resuscitation in a position of cytosine residue and/or size of U3 sequence-specific manner. In addition, the methylation characteristics of the majority of cytosine residues of the different U3 sequences within each size group were conserved. The findings from this study suggest that burn-elicited stress signals contribute to a transient or permanent alteration in cytosine methylation characteristics of certain MuERV loci in the genome, potentially modulating transcription activity of their own as well as neighboring genes.
%K Animals
%K Base Sequence
%K Burns
%K Cytosine
%K DNA Methylation
%K Endogenous Retroviruses
%K Epigenesis, Genetic
%K Female
%K Liver
%K Mice
%K Mice, Inbred C57BL
%K Molecular Sequence Data
%K Polymerase Chain Reaction
%K Proviruses
%K Signal Transduction
%K Stress, Physiological
%U http://dx.doi.org/10.1016/j.yexmp.2010.06.012
%U http://www.ncbi.nlm.nih.gov/pubmed/20609362
%P 291-300

PT Journal
AU Chiu, S
   Hsu, K
   Greenhalgh, DG
   Cho, K
TI Post-injury stress signals alter epigenetic profile of cytosine methylation in the proviral genome of endogenous retroviruses.
SO Experimental and molecular pathology
JI Exp. Mol. Pathol.
PD Dec
PY 2010
BP 291
EP 300
VL 89
IS 3
DI 10.1016/j.yexmp.2010.06.012
LA eng
DE Animals; Base Sequence; Burns; Cytosine; DNA Methylation; Endogenous Retroviruses; Epigenesis, Genetic; Female; Liver; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Polymerase Chain Reaction; Proviruses; Signal Transduction; Stress, Physiological
AB The majority of epigenetic methylation events at cytosine residues of the genome are reported to occur in transposable elements, as a result, it contributes to genome stability by repressing their transposition activity. Our recent studies demonstrated that the expression of certain murine endogenous retroviruses (MuERVs), a family of retrotransposons, is modulated in the liver after burn injury and sepsis. In this study, we investigated whether burn-elicited stress signals alter epigenetic methylation profile of cytosine residues of the MuERV proviral genome. Female C57BL/6J mice were subjected to ~18% total body surface area burn. The genomic DNAs from the livers, which were collected at 3 and 24 h after burn, were treated with bisulfite to convert unmethylated cytosines (C) to thymines (T). From four experimental groups (no burn-3h, burn-3h, no burn-24h, and burn-24h), 91, 98, 94, and 86 unique U3 sequences (from sense or antisense strand) were cloned, respectively and a total of 16 different U3 sizes were identified among them. The survey of C to T conversions in these U3 sequences revealed that the epigenetic profiles of cytosine methylation are differentially affected (increase or decrease in demethylated cytosine residues) by stress signals from burn and/or anesthesia-resuscitation in a position of cytosine residue and/or size of U3 sequence-specific manner. In addition, the methylation characteristics of the majority of cytosine residues of the different U3 sequences within each size group were conserved. The findings from this study suggest that burn-elicited stress signals contribute to a transient or permanent alteration in cytosine methylation characteristics of certain MuERV loci in the genome, potentially modulating transcription activity of their own as well as neighboring genes.
ER

PMID- 20609362
OWN - NLM
STAT- MEDLINE
DA  - 20101115
DCOM- 20101215
LR  - 20131121
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 89
IP  - 3
DP  - 2010 Dec
TI  - Post-injury stress signals alter epigenetic profile of cytosine methylation in
      the proviral genome of endogenous retroviruses.
PG  - 291-300
LID - 10.1016/j.yexmp.2010.06.012 [doi]
AB  - The majority of epigenetic methylation events at cytosine residues of the genome 
      are reported to occur in transposable elements, as a result, it contributes to
      genome stability by repressing their transposition activity. Our recent studies
      demonstrated that the expression of certain murine endogenous retroviruses
      (MuERVs), a family of retrotransposons, is modulated in the liver after burn
      injury and sepsis. In this study, we investigated whether burn-elicited stress
      signals alter epigenetic methylation profile of cytosine residues of the MuERV
      proviral genome. Female C57BL/6J mice were subjected to ~18% total body surface
      area burn. The genomic DNAs from the livers, which were collected at 3 and 24 h
      after burn, were treated with bisulfite to convert unmethylated cytosines (C) to 
      thymines (T). From four experimental groups (no burn-3h, burn-3h, no burn-24h,
      and burn-24h), 91, 98, 94, and 86 unique U3 sequences (from sense or antisense
      strand) were cloned, respectively and a total of 16 different U3 sizes were
      identified among them. The survey of C to T conversions in these U3 sequences
      revealed that the epigenetic profiles of cytosine methylation are differentially 
      affected (increase or decrease in demethylated cytosine residues) by stress
      signals from burn and/or anesthesia-resuscitation in a position of cytosine
      residue and/or size of U3 sequence-specific manner. In addition, the methylation 
      characteristics of the majority of cytosine residues of the different U3
      sequences within each size group were conserved. The findings from this study
      suggest that burn-elicited stress signals contribute to a transient or permanent 
      alteration in cytosine methylation characteristics of certain MuERV loci in the
      genome, potentially modulating transcription activity of their own as well as
      neighboring genes.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Chiu, Sophia
AU  - Chiu S
AD  - Shriners Hospitals for Children Northern California and Department of Surgery,
      University of California, Davis, Sacramento, CA 95817, USA.
FAU - Hsu, Karen
AU  - Hsu K
FAU - Greenhalgh, David G
AU  - Greenhalgh DG
FAU - Cho, Kiho
AU  - Cho K
LA  - eng
GR  - R01GM071360/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100706
PL  - United States
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 8J337D1HZY (Cytosine)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Burns/*genetics
MH  - Cytosine/*metabolism
MH  - DNA Methylation
MH  - Endogenous Retroviruses/*genetics
MH  - *Epigenesis, Genetic
MH  - Female
MH  - Liver/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Proviruses/genetics
MH  - Signal Transduction/genetics
MH  - Stress, Physiological/*genetics
EDAT- 2010/07/09 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/07/09 06:00
PHST- 2010/05/22 [received]
PHST- 2010/06/29 [accepted]
PHST- 2010/07/06 [aheadofprint]
AID - S0014-4800(10)00096-1 [pii]
AID - 10.1016/j.yexmp.2010.06.012 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2010 Dec;89(3):291-300. doi: 10.1016/j.yexmp.2010.06.012. Epub
      2010 Jul 6.
TY  - JOUR
AU  - Chiu, Sophia
AU  - Hsu, Karen
AU  - Greenhalgh, David G.
AU  - Cho, Kiho
PY  - 2010/Dec/06
TI  - Post-injury stress signals alter epigenetic profile of cytosine methylation in the proviral genome of endogenous retroviruses.
T2  - Exp. Mol. Pathol.
JO  - Experimental and molecular pathology
SP  - 291
EP  - 300
VL  - 89
IS  - 3
KW  - Animals
KW  - Base Sequence
KW  - Burns
KW  - Cytosine
KW  - DNA Methylation
KW  - Endogenous Retroviruses
KW  - Epigenesis, Genetic
KW  - Female
KW  - Liver
KW  - Mice
KW  - Mice, Inbred C57BL
KW  - Molecular Sequence Data
KW  - Polymerase Chain Reaction
KW  - Proviruses
KW  - Signal Transduction
KW  - Stress, Physiological
N2  - The majority of epigenetic methylation events at cytosine residues of the genome are reported to occur in transposable elements, as a result, it contributes to genome stability by repressing their transposition activity. Our recent studies demonstrated that the expression of certain murine endogenous retroviruses (MuERVs), a family of retrotransposons, is modulated in the liver after burn injury and sepsis. In this study, we investigated whether burn-elicited stress signals alter epigenetic methylation profile of cytosine residues of the MuERV proviral genome. Female C57BL/6J mice were subjected to ~18% total body surface area burn. The genomic DNAs from the livers, which were collected at 3 and 24 h after burn, were treated with bisulfite to convert unmethylated cytosines (C) to thymines (T). From four experimental groups (no burn-3h, burn-3h, no burn-24h, and burn-24h), 91, 98, 94, and 86 unique U3 sequences (from sense or antisense strand) were cloned, respectively and a total of 16 different U3 sizes were identified among them. The survey of C to T conversions in these U3 sequences revealed that the epigenetic profiles of cytosine methylation are differentially affected (increase or decrease in demethylated cytosine residues) by stress signals from burn and/or anesthesia-resuscitation in a position of cytosine residue and/or size of U3 sequence-specific manner. In addition, the methylation characteristics of the majority of cytosine residues of the different U3 sequences within each size group were conserved. The findings from this study suggest that burn-elicited stress signals contribute to a transient or permanent alteration in cytosine methylation characteristics of certain MuERV loci in the genome, potentially modulating transcription activity of their own as well as neighboring genes.
SN  - 1096-0945
UR  - http://dx.doi.org/10.1016/j.yexmp.2010.06.012
UR  - http://www.ncbi.nlm.nih.gov/pubmed/20609362
ID  - Chiu2010
ER  -