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High-throughput gene expression profiling of memory differentiation in primary human T cells.

Abstract The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and the in vitro expansion of memory cells. However, chemical screens to identify compounds that induce memory differentiation have been limited by 1) the lack of reporter-gene or functional assays that can distinguish naive and memory-phenotype T cells at high throughput and 2) a suitable cell-line representative of naive T cells.
PMID
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Authors

Mayor MeshTerms

Gene Expression Profiling

Keywords
Journal Title bmc immunology
Publication Year Start
%A Haining, W. Nicholas; Angelosanto, Jill; Brosnahan, Kathleen; Ross, Kenneth; Hahn, Cynthia; Russell, Kate; Drury, Linda; Norton, Stephanie; Nadler, Lee; Stegmaier, Kimberly
%T High-throughput gene expression profiling of memory differentiation in primary human T cells.
%J BMC immunology, vol. 9, p. 44
%D 08/2008
%V 9
%M eng
%B The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and the in vitro expansion of memory cells. However, chemical screens to identify compounds that induce memory differentiation have been limited by 1) the lack of reporter-gene or functional assays that can distinguish naive and memory-phenotype T cells at high throughput and 2) a suitable cell-line representative of naive T cells.
%K Antigens, CD31, Antigens, CD95, CD4-Positive T-Lymphocytes, Cell Culture Techniques, Cell Differentiation, Galectin 3, Gene Expression Profiling, Humans, Immunologic Memory, Immunomagnetic Separation, Nucleic Acid Amplification Techniques, Oligonucleotide Array Sequence Analysis, Receptors, CCR7, Reproducibility of Results
%P 44
%Y 10.1186/1471-2172-9-44
%W PHY
%G AUTHOR
%R 2008........9...44H

@Article{Haining2008,
author="Haining, W. Nicholas
and Angelosanto, Jill
and Brosnahan, Kathleen
and Ross, Kenneth
and Hahn, Cynthia
and Russell, Kate
and Drury, Linda
and Norton, Stephanie
and Nadler, Lee
and Stegmaier, Kimberly",
title="High-throughput gene expression profiling of memory differentiation in primary human T cells.",
journal="BMC immunology",
year="2008",
month="Aug",
day="01",
volume="9",
pages="44",
keywords="Antigens, CD31",
keywords="Antigens, CD95",
keywords="CD4-Positive T-Lymphocytes",
keywords="Cell Culture Techniques",
keywords="Cell Differentiation",
keywords="Galectin 3",
keywords="Gene Expression Profiling",
keywords="Humans",
keywords="Immunologic Memory",
keywords="Immunomagnetic Separation",
keywords="Nucleic Acid Amplification Techniques",
keywords="Oligonucleotide Array Sequence Analysis",
keywords="Receptors, CCR7",
keywords="Reproducibility of Results",
abstract="The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and the in vitro expansion of memory cells. However, chemical screens to identify compounds that induce memory differentiation have been limited by 1) the lack of reporter-gene or functional assays that can distinguish naive and memory-phenotype T cells at high throughput and 2) a suitable cell-line representative of naive T cells.",
issn="1471-2172",
doi="10.1186/1471-2172-9-44",
url="http://www.ncbi.nlm.nih.gov/pubmed/18673556",
language="eng"
}

%0 Journal Article
%T High-throughput gene expression profiling of memory differentiation in primary human T cells.
%A Haining, W. Nicholas
%A Angelosanto, Jill
%A Brosnahan, Kathleen
%A Ross, Kenneth
%A Hahn, Cynthia
%A Russell, Kate
%A Drury, Linda
%A Norton, Stephanie
%A Nadler, Lee
%A Stegmaier, Kimberly
%J BMC immunology
%D 2008
%8 August 01
%V 9
%@ 1471-2172
%G eng
%F Haining2008
%X The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and the in vitro expansion of memory cells. However, chemical screens to identify compounds that induce memory differentiation have been limited by 1) the lack of reporter-gene or functional assays that can distinguish naive and memory-phenotype T cells at high throughput and 2) a suitable cell-line representative of naive T cells.
%K Antigens, CD31
%K Antigens, CD95
%K CD4-Positive T-Lymphocytes
%K Cell Culture Techniques
%K Cell Differentiation
%K Galectin 3
%K Gene Expression Profiling
%K Humans
%K Immunologic Memory
%K Immunomagnetic Separation
%K Nucleic Acid Amplification Techniques
%K Oligonucleotide Array Sequence Analysis
%K Receptors, CCR7
%K Reproducibility of Results
%U http://dx.doi.org/10.1186/1471-2172-9-44
%U http://www.ncbi.nlm.nih.gov/pubmed/18673556
%P 44

PT Journal
AU Haining, WN
   Angelosanto, J
   Brosnahan, K
   Ross, K
   Hahn, C
   Russell, K
   Drury, L
   Norton, S
   Nadler, L
   Stegmaier, K
TI High-throughput gene expression profiling of memory differentiation in primary human T cells.
SO BMC immunology
JI BMC Immunol.
PD 08
PY 2008
BP 44
VL 9
DI 10.1186/1471-2172-9-44
LA eng
DE Antigens, CD31; Antigens, CD95; CD4-Positive T-Lymphocytes; Cell Culture Techniques; Cell Differentiation; Galectin 3; Gene Expression Profiling; Humans; Immunologic Memory; Immunomagnetic Separation; Nucleic Acid Amplification Techniques; Oligonucleotide Array Sequence Analysis; Receptors, CCR7; Reproducibility of Results
AB The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and the in vitro expansion of memory cells. However, chemical screens to identify compounds that induce memory differentiation have been limited by 1) the lack of reporter-gene or functional assays that can distinguish naive and memory-phenotype T cells at high throughput and 2) a suitable cell-line representative of naive T cells.
ER

PMID- 18673556
OWN - NLM
STAT- MEDLINE
DA  - 20080905
DCOM- 20081029
LR  - 20140903
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 9
DP  - 2008
TI  - High-throughput gene expression profiling of memory differentiation in primary
      human T cells.
PG  - 44
LID - 10.1186/1471-2172-9-44 [doi]
AB  - BACKGROUND: The differentiation of naive T and B cells into memory lymphocytes is
      essential for immunity to pathogens. Therapeutic manipulation of this cellular
      differentiation program could improve vaccine efficacy and the in vitro expansion
      of memory cells. However, chemical screens to identify compounds that induce
      memory differentiation have been limited by 1) the lack of reporter-gene or
      functional assays that can distinguish naive and memory-phenotype T cells at high
      throughput and 2) a suitable cell-line representative of naive T cells. RESULTS: 
      Here, we describe a method for gene-expression based screening that allows
      primary naive and memory-phenotype lymphocytes to be discriminated based on
      complex genes signatures corresponding to these differentiation states. We used
      ligation-mediated amplification and a fluorescent, bead-based detection system to
      quantify simultaneously 55 transcripts representing naive and memory-phenotype
      signatures in purified populations of human T cells. The use of a multi-gene
      panel allowed better resolution than any constituent single gene. The method was 
      precise, correlated well with Affymetrix microarray data, and could be easily
      scaled up for high-throughput. CONCLUSION: This method provides a generic
      solution for high-throughput differentiation screens in primary human T cells
      where no single-gene or functional assay is available. This screening platform
      will allow the identification of small molecules, genes or soluble factors that
      direct memory differentiation in naive human lymphocytes.
FAU - Haining, W Nicholas
AU  - Haining WN
AD  - Department of Pediatric Dana-Farber Cancer Institute, 44 Binney Street, Boston,
      MA 02115, USA. [email protected]
FAU - Angelosanto, Jill
AU  - Angelosanto J
FAU - Brosnahan, Kathleen
AU  - Brosnahan K
FAU - Ross, Kenneth
AU  - Ross K
FAU - Hahn, Cynthia
AU  - Hahn C
FAU - Russell, Kate
AU  - Russell K
FAU - Drury, Linda
AU  - Drury L
FAU - Norton, Stephanie
AU  - Norton S
FAU - Nadler, Lee
AU  - Nadler L
FAU - Stegmaier, Kimberly
AU  - Stegmaier K
LA  - eng
PT  - Journal Article
DEP - 20080801
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 0 (Antigens, CD31)
RN  - 0 (Antigens, CD95)
RN  - 0 (CCR7 protein, human)
RN  - 0 (FAS protein, human)
RN  - 0 (Galectin 3)
RN  - 0 (Receptors, CCR7)
SB  - IM
MH  - Antigens, CD31/genetics/immunology
MH  - Antigens, CD95/genetics/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation/immunology
MH  - Galectin 3/genetics/*immunology
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Immunologic Memory/*genetics
MH  - Immunomagnetic Separation
MH  - Nucleic Acid Amplification Techniques
MH  - Oligonucleotide Array Sequence Analysis
MH  - Receptors, CCR7/genetics/immunology
MH  - Reproducibility of Results
PMC - PMC2529265
OID - NLM: PMC2529265
EDAT- 2008/08/05 09:00
MHDA- 2008/10/31 09:00
CRDT- 2008/08/05 09:00
PHST- 2008/05/29 [received]
PHST- 2008/08/01 [accepted]
PHST- 2008/08/01 [aheadofprint]
AID - 1471-2172-9-44 [pii]
AID - 10.1186/1471-2172-9-44 [doi]
PST - epublish
SO  - BMC Immunol. 2008 Aug 1;9:44. doi: 10.1186/1471-2172-9-44.
TY  - JOUR
AU  - Haining, W. Nicholas
AU  - Angelosanto, Jill
AU  - Brosnahan, Kathleen
AU  - Ross, Kenneth
AU  - Hahn, Cynthia
AU  - Russell, Kate
AU  - Drury, Linda
AU  - Norton, Stephanie
AU  - Nadler, Lee
AU  - Stegmaier, Kimberly
PY  - 2008/08/01
TI  - High-throughput gene expression profiling of memory differentiation in primary human T cells.
T2  - BMC Immunol.
JO  - BMC immunology
SP  - 44
VL  - 9
KW  - Antigens, CD31
KW  - Antigens, CD95
KW  - CD4-Positive T-Lymphocytes
KW  - Cell Culture Techniques
KW  - Cell Differentiation
KW  - Galectin 3
KW  - Gene Expression Profiling
KW  - Humans
KW  - Immunologic Memory
KW  - Immunomagnetic Separation
KW  - Nucleic Acid Amplification Techniques
KW  - Oligonucleotide Array Sequence Analysis
KW  - Receptors, CCR7
KW  - Reproducibility of Results
N2  - The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and the in vitro expansion of memory cells. However, chemical screens to identify compounds that induce memory differentiation have been limited by 1) the lack of reporter-gene or functional assays that can distinguish naive and memory-phenotype T cells at high throughput and 2) a suitable cell-line representative of naive T cells.
SN  - 1471-2172
UR  - http://dx.doi.org/10.1186/1471-2172-9-44
UR  - http://www.ncbi.nlm.nih.gov/pubmed/18673556
ID  - Haining2008
ER  - 
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