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Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages.

Abstract Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title journal of virology
Publication Year Start
%A Ryzhova, Elena V.; Vos, Robin M.; Albright, Andrew V.; Harrist, Alexia V.; Harvey, Thomas; Gonz?lez-Scarano, Francisco
%T Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages.
%J Journal of virology, vol. 80, no. 6, pp. 2694-2704
%D 03/2006
%V 80
%N 6
%M eng
%B Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.
%K Annexin A2, Antigens, CD, Antigens, CD63, Cells, Cultured, Gene Products, gag, Genes, gag, HIV-1, Humans, Macrophages, Platelet Membrane Glycoproteins, Protein Precursors, Virus Assembly, Virus Replication
%P 2694
%L 2704
%Y 10.1128/JVI.80.6.2694-2704.2006
%W PHY
%G AUTHOR
%R 2006.......80.2694R

@Article{Ryzhova2006,
author="Ryzhova, Elena V.
and Vos, Robin M.
and Albright, Andrew V.
and Harrist, Alexia V.
and Harvey, Thomas
and Gonz{\'a}lez-Scarano, Francisco",
title="Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages.",
journal="Journal of virology",
year="2006",
month="Mar",
volume="80",
number="6",
pages="2694--2704",
keywords="Annexin A2",
keywords="Antigens, CD",
keywords="Antigens, CD63",
keywords="Cells, Cultured",
keywords="Gene Products, gag",
keywords="Genes, gag",
keywords="HIV-1",
keywords="Humans",
keywords="Macrophages",
keywords="Platelet Membrane Glycoproteins",
keywords="Protein Precursors",
keywords="Virus Assembly",
keywords="Virus Replication",
abstract="Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.",
issn="0022-538X",
doi="10.1128/JVI.80.6.2694-2704.2006",
url="http://www.ncbi.nlm.nih.gov/pubmed/16501079",
language="eng"
}

%0 Journal Article
%T Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages.
%A Ryzhova, Elena V.
%A Vos, Robin M.
%A Albright, Andrew V.
%A Harrist, Alexia V.
%A Harvey, Thomas
%A Gonz?lez-Scarano, Francisco
%J Journal of virology
%D 2006
%8 Mar
%V 80
%N 6
%@ 0022-538X
%G eng
%F Ryzhova2006
%X Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.
%K Annexin A2
%K Antigens, CD
%K Antigens, CD63
%K Cells, Cultured
%K Gene Products, gag
%K Genes, gag
%K HIV-1
%K Humans
%K Macrophages
%K Platelet Membrane Glycoproteins
%K Protein Precursors
%K Virus Assembly
%K Virus Replication
%U http://dx.doi.org/10.1128/JVI.80.6.2694-2704.2006
%U http://www.ncbi.nlm.nih.gov/pubmed/16501079
%P 2694-2704

PT Journal
AU Ryzhova, EV
   Vos, RM
   Albright, AV
   Harrist, AV
   Harvey, T
   Gonz?lez-Scarano, F
TI Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages.
SO Journal of virology
JI J. Virol.
PD Mar
PY 2006
BP 2694
EP 2704
VL 80
IS 6
DI 10.1128/JVI.80.6.2694-2704.2006
LA eng
DE Annexin A2; Antigens, CD; Antigens, CD63; Cells, Cultured; Gene Products, gag; Genes, gag; HIV-1; Humans; Macrophages; Platelet Membrane Glycoproteins; Protein Precursors; Virus Assembly; Virus Replication
AB Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.
ER

PMID- 16501079
OWN - NLM
STAT- MEDLINE
DA  - 20060227
DCOM- 20060405
LR  - 20140909
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 6
DP  - 2006 Mar
TI  - Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein
      involved in replication in monocyte-derived macrophages.
PG  - 2694-704
AB  - Human immunodeficiency virus (HIV) replication in the major natural target cells,
      CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life
      cycle. However, it differs as to viral assembly and budding, which take place on 
      plasma membranes in T cells and on endosomal membranes in macrophages. It has
      been postulated that cell type-specific host factors may aid in directing viral
      assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a
      novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to
      productively infected macrophages and was not detected in quiescently infected
      monocyte-derived macrophages (MDM) in which an HIV replication block was mapped
      to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F.
      Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag
      interaction likely occurs at the limiting membranes of late
      endosomes/multivesicular bodies and that Anx2 depletion is associated with a
      significant decline in the infectivity of released virions; this coincided with
      incomplete Gag processing and inefficient incorporation of CD63. Cumulatively,
      our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.
FAU - Ryzhova, Elena V
AU  - Ryzhova EV
AD  - Department of Neurology and Microbiology, University of Pennsylvania, 3 W. Gates,
      3400 Spruce Street, Philadelphia, Pennsylvania 19104-4283, USA.
FAU - Vos, Robin M
AU  - Vos RM
FAU - Albright, Andrew V
AU  - Albright AV
FAU - Harrist, Alexia V
AU  - Harrist AV
FAU - Harvey, Thomas
AU  - Harvey T
FAU - Gonzalez-Scarano, Francisco
AU  - Gonzalez-Scarano F
LA  - eng
GR  - NS-27405/NS/NINDS NIH HHS/United States
GR  - NS-35743/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (ANXA2 protein, human)
RN  - 0 (Annexin A2)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD63)
RN  - 0 (CD63 protein, human)
RN  - 0 (Gene Products, gag)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Protein Precursors)
RN  - 0 (p55 gag precursor protein, Human immunodeficiency virus 1)
SB  - IM
MH  - Annexin A2/*metabolism
MH  - Antigens, CD/metabolism
MH  - Antigens, CD63
MH  - Cells, Cultured
MH  - Gene Products, gag/*metabolism
MH  - Genes, gag
MH  - HIV-1/metabolism/*pathogenicity/*physiology
MH  - Humans
MH  - Macrophages/*virology
MH  - Platelet Membrane Glycoproteins/metabolism
MH  - Protein Precursors/metabolism
MH  - Virus Assembly
MH  - Virus Replication
PMC - PMC1395445
OID - NLM: PMC1395445
EDAT- 2006/02/28 09:00
MHDA- 2006/04/06 09:00
CRDT- 2006/02/28 09:00
AID - 80/6/2694 [pii]
AID - 10.1128/JVI.80.6.2694-2704.2006 [doi]
PST - ppublish
SO  - J Virol. 2006 Mar;80(6):2694-704.
TY  - JOUR
AU  - Ryzhova, Elena V.
AU  - Vos, Robin M.
AU  - Albright, Andrew V.
AU  - Harrist, Alexia V.
AU  - Harvey, Thomas
AU  - Gonz?lez-Scarano, Francisco
PY  - 2006/Mar/
TI  - Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages.
T2  - J. Virol.
JO  - Journal of virology
SP  - 2694
EP  - 2704
VL  - 80
IS  - 6
KW  - Annexin A2
KW  - Antigens, CD
KW  - Antigens, CD63
KW  - Cells, Cultured
KW  - Gene Products, gag
KW  - Genes, gag
KW  - HIV-1
KW  - Humans
KW  - Macrophages
KW  - Platelet Membrane Glycoproteins
KW  - Protein Precursors
KW  - Virus Assembly
KW  - Virus Replication
N2  - Human immunodeficiency virus (HIV) replication in the major natural target cells, CD4+ T lymphocytes and macrophages, is parallel in many aspects of the virus life cycle. However, it differs as to viral assembly and budding, which take place on plasma membranes in T cells and on endosomal membranes in macrophages. It has been postulated that cell type-specific host factors may aid in directing viral assembly to distinct destinations. In this study we defined annexin 2 (Anx2) as a novel HIV Gag binding partner in macrophages. Anx2-Gag binding was confined to productively infected macrophages and was not detected in quiescently infected monocyte-derived macrophages (MDM) in which an HIV replication block was mapped to the late stages of the viral life cycle (A. V. Albright, R. M. Vos, and F. Gonzalez-Scarano, Virology 325:328-339, 2004). We demonstrate that the Anx2-Gag interaction likely occurs at the limiting membranes of late endosomes/multivesicular bodies and that Anx2 depletion is associated with a significant decline in the infectivity of released virions; this coincided with incomplete Gag processing and inefficient incorporation of CD63. Cumulatively, our data suggest that Anx2 is essential for the proper assembly of HIV in MDM.
SN  - 0022-538X
UR  - http://dx.doi.org/10.1128/JVI.80.6.2694-2704.2006
UR  - http://www.ncbi.nlm.nih.gov/pubmed/16501079
ID  - Ryzhova2006
ER  - 
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