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Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons.

Abstract The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title the journal of comparative neurology
Publication Year Start
%A Beech, Robert D.; Cleary, Muriel A.; Treloar, Helen B.; Eisch, Amelia J.; Harrist, Alexia V.; Zhong, Weimin; Greer, Charles A.; Duman, Ronald S.; Picciotto, Marina R.
%T Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons.
%J The Journal of comparative neurology, vol. 475, no. 1, pp. 128-141
%D 07/2004
%V 475
%N 1
%M eng
%B The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.
%K Animals, Cyclic AMP Response Element-Binding Protein, Gene Expression Regulation, Intermediate Filament Proteins, Mice, Mice, Transgenic, Nerve Tissue Proteins, Nestin, Neurons, Olfactory Bulb, Promoter Regions, Genetic, Stem Cells, Transgenes
%P 128
%L 141
%Y 10.1002/cne.20179
%W PHY
%G AUTHOR
%R 2004......475..128B

@Article{Beech2004,
author="Beech, Robert D.
and Cleary, Muriel A.
and Treloar, Helen B.
and Eisch, Amelia J.
and Harrist, Alexia V.
and Zhong, Weimin
and Greer, Charles A.
and Duman, Ronald S.
and Picciotto, Marina R.",
title="Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons.",
journal="The Journal of comparative neurology",
year="2004",
month="Jul",
day="12",
volume="475",
number="1",
pages="128--141",
keywords="Animals",
keywords="Cyclic AMP Response Element-Binding Protein",
keywords="Gene Expression Regulation",
keywords="Intermediate Filament Proteins",
keywords="Mice",
keywords="Mice, Transgenic",
keywords="Nerve Tissue Proteins",
keywords="Nestin",
keywords="Neurons",
keywords="Olfactory Bulb",
keywords="Promoter Regions, Genetic",
keywords="Stem Cells",
keywords="Transgenes",
abstract="The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.",
issn="0021-9967",
doi="10.1002/cne.20179",
url="http://www.ncbi.nlm.nih.gov/pubmed/15176089",
language="eng"
}

%0 Journal Article
%T Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons.
%A Beech, Robert D.
%A Cleary, Muriel A.
%A Treloar, Helen B.
%A Eisch, Amelia J.
%A Harrist, Alexia V.
%A Zhong, Weimin
%A Greer, Charles A.
%A Duman, Ronald S.
%A Picciotto, Marina R.
%J The Journal of comparative neurology
%D 2004
%8 Jul 12
%V 475
%N 1
%@ 0021-9967
%G eng
%F Beech2004
%X The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.
%K Animals
%K Cyclic AMP Response Element-Binding Protein
%K Gene Expression Regulation
%K Intermediate Filament Proteins
%K Mice
%K Mice, Transgenic
%K Nerve Tissue Proteins
%K Nestin
%K Neurons
%K Olfactory Bulb
%K Promoter Regions, Genetic
%K Stem Cells
%K Transgenes
%U http://dx.doi.org/10.1002/cne.20179
%U http://www.ncbi.nlm.nih.gov/pubmed/15176089
%P 128-141

PT Journal
AU Beech, RD
   Cleary, MA
   Treloar, HB
   Eisch, AJ
   Harrist, AV
   Zhong, W
   Greer, CA
   Duman, RS
   Picciotto, MR
TI Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons.
SO The Journal of comparative neurology
JI J. Comp. Neurol.
PD Jul
PY 2004
BP 128
EP 141
VL 475
IS 1
DI 10.1002/cne.20179
LA eng
DE Animals; Cyclic AMP Response Element-Binding Protein; Gene Expression Regulation; Intermediate Filament Proteins; Mice; Mice, Transgenic; Nerve Tissue Proteins; Nestin; Neurons; Olfactory Bulb; Promoter Regions, Genetic; Stem Cells; Transgenes
AB The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.
ER

PMID- 15176089
OWN - NLM
STAT- MEDLINE
DA  - 20040603
DCOM- 20040715
LR  - 20131121
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 475
IP  - 1
DP  - 2004 Jul 12
TI  - Nestin promoter/enhancer directs transgene expression to precursors of adult
      generated periglomerular neurons.
PG  - 128-41
AB  - The subventricular zone (SVZ) is a major neurogenic region in the adult brain.
      Cells from the SVZ give rise to two populations of olfactory bulb interneurons:
      the granule cells and periglomerular (PG) cells. Currently, little is known about
      the signaling pathways that direct these newly generated neurons to become either
      granule or PG neurons. In the present study, we used the nestin promoter and
      enhancer to direct expression of the tetracycline transactivator (tTA). We
      generated two independent strains of nestin-tTA transgenic animals and crossed
      founder mice from both lines to mice containing a tetracycline-regulated
      transgene (mCREB) whose expression served as a marker for the activity of the
      nestin-tTA transgene. mCREB expression occurred in a subset of proliferating
      cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in
      both lines of nestin-tTA mice transgene expression in the olfactory bulb was
      limited to PG neurons and was absent from granule cells, suggesting that this
      nestin promoter construct differentiates between the two interneuronal
      populations. Transgene expression occurred in several subtypes of PG neurons,
      including those expressing calretinin, calbindin, GAD67, and tyrosine
      hydroxylase. These results suggest that a unique subset of SVZ precursor cells
      gives rise to PG, and not granule cells. The ability to express different
      transgenes within this subpopulation of neuronal precursors provides a powerful
      system to define the signals regulating the differentiation and survival of
      adult-generated neurons in the olfactory bulb.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Beech, Robert D
AU  - Beech RD
AD  - Department of Psychiatry, Yale University School of Medicine, 34 Park Street, 3rd
      Floor, New Haven, CT 06508, USA. [email protected]
FAU - Cleary, Muriel A
AU  - Cleary MA
FAU - Treloar, Helen B
AU  - Treloar HB
FAU - Eisch, Amelia J
AU  - Eisch AJ
FAU - Harrist, Alexia V
AU  - Harrist AV
FAU - Zhong, Weimin
AU  - Zhong W
FAU - Greer, Charles A
AU  - Greer CA
FAU - Duman, Ronald S
AU  - Duman RS
FAU - Picciotto, Marina R
AU  - Picciotto MR
LA  - eng
GR  - DA 00167/DA/NIDA NIH HHS/United States
GR  - MH 25642/MH/NIMH NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nes protein, mouse)
RN  - 0 (Nestin)
SB  - IM
MH  - Animals
MH  - Cyclic AMP Response Element-Binding Protein/biosynthesis/genetics
MH  - Gene Expression Regulation/physiology
MH  - Intermediate Filament Proteins/biosynthesis/*genetics/physiology
MH  - Mice
MH  - Mice, Transgenic
MH  - Nerve Tissue Proteins/biosynthesis/*genetics/physiology
MH  - Nestin
MH  - Neurons/cytology/*metabolism
MH  - Olfactory Bulb/*cytology/metabolism
MH  - Promoter Regions, Genetic/*physiology
MH  - Stem Cells/metabolism/*physiology
MH  - Transgenes/*physiology
EDAT- 2004/06/04 05:00
MHDA- 2004/07/16 05:00
CRDT- 2004/06/04 05:00
AID - 10.1002/cne.20179 [doi]
PST - ppublish
SO  - J Comp Neurol. 2004 Jul 12;475(1):128-41.
TY  - JOUR
AU  - Beech, Robert D.
AU  - Cleary, Muriel A.
AU  - Treloar, Helen B.
AU  - Eisch, Amelia J.
AU  - Harrist, Alexia V.
AU  - Zhong, Weimin
AU  - Greer, Charles A.
AU  - Duman, Ronald S.
AU  - Picciotto, Marina R.
PY  - 2004/Jul/12
TI  - Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons.
T2  - J. Comp. Neurol.
JO  - The Journal of comparative neurology
SP  - 128
EP  - 141
VL  - 475
IS  - 1
KW  - Animals
KW  - Cyclic AMP Response Element-Binding Protein
KW  - Gene Expression Regulation
KW  - Intermediate Filament Proteins
KW  - Mice
KW  - Mice, Transgenic
KW  - Nerve Tissue Proteins
KW  - Nestin
KW  - Neurons
KW  - Olfactory Bulb
KW  - Promoter Regions, Genetic
KW  - Stem Cells
KW  - Transgenes
N2  - The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.
SN  - 0021-9967
UR  - http://dx.doi.org/10.1002/cne.20179
UR  - http://www.ncbi.nlm.nih.gov/pubmed/15176089
ID  - Beech2004
ER  - 
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